Effects of Medicinal Serum of Shenqi Yiliu Formula on Proliferation of Gastric Cancer MGC-803 Cells / 中国中医药信息杂志

ABSTRACT

Objective To investigate the inhibitory effects of medicinal serum of Shenqi Yiliu Formula on cell proliferation of gastric cancer MGC-803 cells;To discuss relevant mechanism. Methods After treated with different concentrations of medicinal serum of Shenqi Yiliu Formula, gastric cancer MGC-803 cells were tested by the following methods: MTT was employed to test the proliferation of gastric cancer MGC-803 cells; Flow cytometry was used to detect cell cycle; qRT-PCR was used to detect the genetic expressions of CDKN1B and CDKN1C;Western blot was employed to test the protein expressions of p27 and p57. Results When 3%, 5%and 10%medicinal serum of Shenqi Yiliu Formula was treated to MGC-803 cells for 24 h, 48 h and 72 h, proliferation of cells decreased significantly (P<0.01), with time- and dosage-dependent relationship. When 3%, 5% and 10% medicinal serum of Shenqi Yiliu Formula was treated to MGC-803 cells for 24 h, cells in G0/G1 increased, decreased in S. qRT-PCR results showed that compared with the blank control group and the negative control group, mRNA expressions of CDKN1B and CDKN1C of MGC-803 cells in medicinal serum all dose group increased significantly (P<0.01). Western blot results showed that compared with the blank control group, protein expressions of p27 and p57 of MGC-803 cells in medicinal serum all dose group increased significantly (P<0.01). Conclusion Medicinal serum of Shenqi Yiliu Formula can inhibit MGC-803 cells proliferation and the mechanism may be through adjusting CDKN1B, CDKN1C mRNA and proteins expression to intervene in the cell cycle.