Research in immunological characteristics of recombinant Rv3425 protein of my-cobacterium tuberculosis / 中国免疫学杂志

ABSTRACT

Objective:To study the immunological characteristics of recombinant Rv3425 protein,to evaluate its diagnosis value and the role in the pathogenicity. Methods: Rv3425 gene was cloned into pET28a vector,the recombinant protein was induced and purified;we analyzed the antigenicity and specificity of Rv3425 by ELISA ( Enzyme-linked immuno sorbent assay) and Western blot methods,apoptosis effect of Rv3425 to macrophage was deteced by FCM ( Flow cytometry method ) . Results: Purified prokaryotic expressed protein Rv3425 was acquired,we found Rv3425 could elicit high level of IFN-γin spleen cells and combine with the serum of iH37Rv (inactivated mycobacterium tuberculosis) immunized mice;the specific IgG and IgM antibodies of TB (Tuberculosis) patients were significantly higher than healthy donors;Rv3425 also could induce the necrosis of macrophage. Conclusion:Our study found that Rv3425 had strong antigenicity and could induce the apoptosis of macrophage,these findings were very important for the research of TB diagnosis and pathogenicity.