Bioinformatics characteristics of lncRNA -uc.167 and its temporal and spatial expression pattern for mouse embryonic development / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the basic biological characteristics of lncRNA -uc.1 67,and its spatial dis-tribution,temporal expression pattern during the mouse embryonic development.Methods The UCSC genome browser of ENCODE was used to analyze preliminary bioinformatics of lncRNAs.Real -time (RT)-PCR was applied to detect the expression of uc.1 67 and neighboring genes in the embryonic mouse heart in different stages (P7.5,P1 1 .5,P1 4.5, P1 8.5).Dimethyl sulphoxide was used to induce P1 9 cell differentiation into the cardiomyocytes.RT -PCR was applied to detect the expression changes in uc.1 67 and neighboring genes on differential day 0,4,6,8 and 1 0.Results Full -length of human uc.167 was 201 bp,and human uc.167 was located in the genome 5q14.3 (chr588179623 -881 79824,GRCh37 /hg1 9).uc.1 67 mainly expressed in the ventricular muscle tissue.The expression of uc.1 67 was gradually decreased in the mouse embryonic heart development process(P7.51 .000 ±0.1 00,P1 1 .50.71 4 ±0.1 07, P1 4.50.393 ±0.043,P1 8.50.1 25 ±0.01 3),while the expression of its neighboring Mef2c gene was gradually in-creased(P7.51 .081 ±0.1 1 8,P1 1 .52.340 ±0.351 ,P1 4.53.958 ±0.542,P1 8.59.361 ±0.722),which showed an opposite trend.The expression of uc.1 67 during P1 9 cell differentiation into cardiomyocytes showed a an increase at first and then a decreasepattern,and the highest level expression of uc.1 67 was on differential day 4(d01 .071 ± 0.1 1 7,d44.71 4 ±0.501 ,d63.572 ±0.41 4,d82.550 ±0.31 4,d1 00.786 ±0.085).The expression of neigh-boring gene Mef2c was in an opposite trend(d01 .01 2 ±0.041 ,d40.353 ±0.037,d62.470 ±0.329,d86.706 ± 0.682,d1 07.765 ±0.705).Conclusions It is suggested that uc.1 67 may take part in the process of embryonic heart development and may play a role through negatively regulating its neighboring gene Mef2c.