Identification and quantification of flavonoids in Carica papaya leaf and peroxynitrite-scavenging activity
Asian Pacific Journal of Tropical Biomedicine
;
(12): 208-213, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-511489
ABSTRACT
Objective:
To characterize the types, contents, and peroxynitrite-scavenging activities of flavonoids in the leaf of Carica papaya (C. papaya).Methods:
Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis and peroxynitrite-scavenging assay were performed to isolate and quantify flavonoid compounds in the flavonoid-rich fraction (BuOH fraction) derived from MeOH extract of C. papaya leaves and evaluate their peroxynitrite-scavenging activities.Results:
Seven flavonoids were isolated from the leaves of C. papaya, including quer-cetin 3-(2G-rhamnosylrutinoside), kaempferol 3-(2G-rhamnosylrutinoside), quercetin 3-rutinoside, myricetin 3-rhamnoside, kaempferol 3-rutinoside, quercetin, and kaemp-ferol. All of the substances exhibited potent activities on peroxynitrite scavenging (IC50 ≤ 4.15 μmol/L), which were stronger than the positive control, L-penicillamine (6.90μmol/L). The content of kaempferol 3-(2G-rhamnosylrutinoside) was significantly higher than other identified compounds (123.18 mg/g BuOH fraction and 7.23 mg/g MeOH extract).Conclusions:
The results of the present study demonstrate the potent antioxidant fla-vonoids of C. papaya leaf, with kaempferol 3-(2G-rhamnosylrutinoside) as the major one.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Asian Pacific Journal of Tropical Biomedicine
Année:
2017
Type:
Article
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