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Determination of Triptolide in Shenshe Ointment by LC-MS/MS / 中国药师
China Pharmacist ; (12): 745-748, 2017.
Article Dans Chinois | WPRIM | ID: wpr-511648
ABSTRACT

Objective:

To establish an LC-MS/MS method for the content determination of triptolide in Shenshe ointment.

Methods:

The chromatographic separation was performed on an Agilent TC-C18 (250 mm×4.6 mm,5 μm)column with the mobile phase consisting of methanol-0.1% formic acid solution (73∶27,v/v).The flow rate was 0.5 ml·min-1 and the column temperature was maintained at 35℃.An electrospray ionization (ESI) source was applied with multiple reaction monitoring (MRM) combined with a positive mode.Metronidazole was used as the internal standard.The mass transitions were 361.4→43.2 for triptolide and 172.1→128.0 for metronidazole,respectively.

Results:

The linear range was 10-500 ng·ml-1 with good correlation coefficient (r=0.999 9).The limit of quantification (LOQ) was 9.5 ng·ml-1.The intra-and inter-day RSDs of peak areas were all less than 3% and the average recovery was 96.87%(RSD=2.79,n=6).

Conclusion:

The established LC-MS/MS method is simple,efficient,sensitive and accurate in the quality control of Shenshe ointment.

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: China Pharmacist Année: 2017 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) langue: Chinois Texte intégral: China Pharmacist Année: 2017 Type: Article