Expression levels of HIF-1 α and mRNA and MUC5AC,MUC5B mRNA in chronic rhinosinusitis and their correlation / 国际检验医学杂志

ABSTRACT

Objective To explore the expression levels of HIF-1 α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis(CRS) and their correlation.Methods Eighty cases of CRS with nasal polyps(CRSwNP) treated in our hospital from May 2014 to May 2015,80 cases of CRS without NP(CRSsNP) and 80 healthy people without CRS(control group) were chosen.Nasal sinus mucosa in 3 groups were collected and treated.The expressions of HIF-1 α,MUC5AC and MUC5B mRNA were measured by semiquantitative reverse transcription polymerase chain reaction(RT-PCR),and their correlations were analyzed.Results The relative expression levels of HIF-1 α,MUC5AC and MUC5B mRNA in the CRSwNP group or CRSsNP group were 1.35±0.85,1.35±0.91;0.80±0.55,0.79±0.49 and 1.18±1.01,1.21±1.02 respectively,while which in the control group were 0.42±0.33,0.43±0.36 and 0.47±0.43 respectively,the difference between the CRS groups and control group was statistically significant(P<0.05).The expression of HIF-1αin the CRSwNP group and CRSsNP group was positively correlated with MUC5AC,MUC 5B mRNA(r=0.476,P=0.023,r=0.476,P=0.026,r=0.478,P=0.035,r=0.508,P=0.021).The ROC analysis results showed AUC=0.956 4.Conclusion The expression levels of HIF-1α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis are significantly upregulated,moreover HIF-1α is closely related with MUC5AC and MUC5B mRNA.