Application of stable isotope dilution liquid chromatography-mass spectrometry in medical research / 国际药学研究杂志

ABSTRACT

Stable isotope dilution mass spectrometry(ID-MS)is a quantitative method for determining the content of unla?beled compounds with stable isotope labeled compounds as internal standard. Stable isotope dilution liquid chromatography-mass spec?trometry(ID-LC-MS)has the advantages of high sensitivity,no quantitative separation of the analytes,and effective elimination of the biological matrices. The method has been widely used in quantitative analysis of biomarkers,drugs and their metabolites. In this pa?per,the mechanism and characteristics of ID-LC-MS are briefly introduced,and its medical applications are summarized.