Studies on cell surface markers and immunological classification of lymphocytic leukemia and non-Hodgkin's lymphoma:Ⅰ. The establishment and evaluation of a combined E and YC rosette formation test-A screening method for immunological classifica / 中国病理生理杂志

ABSTRACT

Using complement coated Baker's yeast as a ligand to identify complement -receptor bearing cells and E-rosette forming technique, a combined E and YC rosette formation test was established. This technique is a simple useful method to detect E~+, YC~+ and D cells, and also monocytes or phagocytes simutaneously in a single test. The application of this technique to 81 adult peripheral blood and 13 cord blood samples demonstrated that a mean of 61.9 per 100 lymphocytes ( ?10.9) formed E-rosettes, 8.2 ?3.4% formed YC-rosettes and 1.07?1.27% was D cells. The application to immunological classification of the perpheral blood lymphocytes of 12 patients with acute lymphocytic leukemia disclosed a case of B-cell lymphosarcoma-leukemia, a case of T cell leukemia, 4 cases of non-E~+ non-YC~+ lymphocytic leukemia and a case of mixed T cell-non-T non-B lymphocytic leukemia. The other 5 cases appeared to have malignant cells with different markers and need further studies.