Reconstitution of Arterial Wall Using Cell Culture Technique / 昆明医科大学学报

ABSTRACT

Using cell culture technique, in vitro an artificial arterial wall has been constructed by adding cultured endothelial cells derived from the human umbilical vein to the smooth muscle cells isolated from the human umbilical artery. On the condition of vit C and the endothelial cell growth factor in the medium, both the endothelial cells and smooth muscle cells grew very well and formed living arterial walls with the ability of generation and metabolism. A confluent monolayer of flat endothelial cells and multilayer of smooth muscle cells were observed under the light and electron microscope. Among these cells were plenty of collagenous fiber, elastic fiber and glycoproteins produced by the smooth muscle cells. This blood vessel wall model can be used for studying the physiological and pathological process of arteries.