Gene Amplification Test of Mycobacterium Leprae in Paraffin- Embedded Tissue / 中华皮肤科杂志
Chinese Journal of Dermatology
; (12)2000.
Article
de Zh
| WPRIM
| ID: wpr-517379
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To establish a polymerase chain reaction(PCR) method to amplify DNA of M.leprae from fixed and paraffin- embedded tissue(FPET). Methods The DNA of M.leprae was released from FPET by using Texpat Kit and purified with 100% alcohol. The primers RPOT(1) and RPUT(2) were used to conduct the PCR. Results A total of 32 samples were examined. Out of 32 samples with BI of more than 1+ , 28 were positive for PCR. The PCR was negative in a sample with BI=0. The sensitivity of PCR reached a level of 0.04 pg DNA. Conclusion This PCR method is very useful for amplifying the DNA of M.leprae from FPET.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Dermatology
Année:
2000
Type:
Article