Determination of the Concentration of Midazolam in Plasma by RP-HPLC-UV Detection / 中国药房
China Pharmacy
;
(12)1991.
Article
Dans Chinois
| WPRIM
| ID: wpr-518790
ABSTRACT
OBJECTIVE:
To build up a method of determining the concentration of midazolam in plasma by RPHPLC-UV detection.METHODS:
The separation was carried out by a reversed-phase Hypersil ODS column(250mm?4.0mm,5?m) with a mobile phase consisting of methanol-acetonitrile-0.02mol/L potassium phosphate buffer(pH 7.4) (65∶25∶10,V/V).Mida_zolam was extracted from alkalinizing plasma and soluted in the mobile phase then detected at 221nm.RESULTS:
The calibration curve had the fine linearity in the concentration range 50~1 600ng/ml(r=0.9 999).The detection limit was 2ng/ml.The absolute recovery was 90.8%~95.4%,the relative recovery was 99.3%~101.3%.The within-day and between-day precision(CV%) was 1.94%~5.16%,3.00%~6.39% respectively.CONCLUSION:
The method is simple,stable and highly sensitive and could meet with the research of clinical pharmacokinetics.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
1991
Type:
Article
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