The inhibition by polymorphonuclear leukocytes of TNF-? release from human mononuclear cells and its mechanism / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of human polymorphonuclear leukocytes (PMNs) on the release of TNF-? by the human peripheral blood mononuclear cells (PBMCs) and to elucidate its mechanism. METHODS: Human PMNs and PBMCs were isolated from the venous blood of healthy donors by dextran sedimentation and density gradient centrifugation. After the cells were cocultured at the ratio of 21 in the presence of lipopolysaccharide (LPS), the concentration of TNF-? in the supernatant was measured by enzyme-linked immunosorbent assay. The binding rate of monocytes with the fluorescein isothiocyanate-labeled LPS (FITC-LPS) and the mean surface fluorescence intensity of monocytes were analyzed by flow cytometry. RESULTS: PMNs do not produce detectable TNF-? in the presence of LPS. PMNs were capable of inhibiting the TNF-? release from PBMCs ( P