Effect of activation of stimulator cells on expression of CD69 by responder T cells in mixed lymphocyte reaction / 中国病理生理杂志

ABSTRACT

AIM: To study the influence of status of stimulator cells on activation of responder T cells in mixed lymphocyte reaction (MLR), so as to provide some basis for clinical transplantation. METHODS: Stimulator cells were pretreated differently before mixed lymphocyte culture (MLC) to change their functional status, fluorescence conjugated antibodies and flow cytometry were used to detect expression of CD69 by responder T cells at several different time points. RESULTS: The expression percentages of CD69 by responder T cells in MLCa group (stimulator cells were pre-activated) were significantly higher than those in MLC group (stimulator cells were not pre-activated) at 24, 48 and 72 hours of culture, respectively (5 21%?0 24% vs 1 98%?0 33%, 29 81%?0 85% vs 20 65%?1 00% and 39 61%?1 62% vs 13 49%?0 60%, P