Simultaneous determination of lidocaine and bupivacaine in human spinal cord pretreated with formaldehyde by high performance liquid chromatography / 中国法医学杂志

ABSTRACT

Objective For purpose of meeting the requirement of forensic toxicological investigation, a RPHPLC method was established for simultaneous determining lidocaine and bupivacaine in human spinal cord pretreated with formaldehyde. Method Analytical column was YWG-C18 (4.6mm?150mm) and a per column. Mobile phase was CH3OH0.015M NaH2PO4 =7525 (v/v) pH = 7.2. The wavelength of detection was 210 nm. The pretreatment method of sample, detection condition, linear range, precision and recorery of the method were systematically investigated by using blank spinal cord spiked with standard lidocaine and bupivacaine. Results The linear range was 0.5 ~ 10.0?g.g-1 (lidocaine r=0.9999; bupivacaine r = 0.9998). The detection limit of lidocaine was 15ng and of bupivacaine 20ng (S/N≥3). The intra and inter day precision of assay of lidocaine and bupivacaine were less than 4.3% (n=5). Both lidocaine and bupivacaine have been detected in a forensic toxicological analysis case by using this method and the result was correct. Conclusion Lidocaine and bupivacaine can determined in human spinal cord pretreat-edwith formaldehyde by HPLC. The method is simple, useful and accurate. It can be applied in the forensic toxicological analysis investigation and other medical studies.