Effect of hypoxia on the expression of proliferating cell nuclear antigen and phenotype of cardiac fibroblasts / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-521508
ABSTRACT
AIM:
To investigate effect of hypoxia on the expression of proliferating cell nuclear antigen(PCNA) a nd phenotype of cardiac fibroblasts(CFs).METHODS:
The purif ied cardiac fibro blasts were cultured and divided randomly into there groupscontrol group, mode rate hypoxia(MH) group and severe hypoxia(SH) group. After 72 h,MTT method was u s ed to investigate the proliferation of CFs, and the ultrastructure of fibroblast s were observed with transmission electron microscopy The expression of PCNA a n d ?-actin in cardiac fibroblasts were measured by the means of immunohistochemi s try and laser scanning confocal microscopy, respectively.RESULTS:
MTT A 490 nm value of MH group was significantly higher than that of control group by (18 4?25 0)% ( P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Pathophysiology
Année:
2000
Type:
Article
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