The Effect of Ion-irradiation and Active Macrophage on Vascular Endothelial Cell / 中国医师杂志

ABSTRACT

Objective To investigate the effect of strong ion-irradiation and active macrophage on vascular endothelial cells. Methods The vascular endothelial cells(ECV-304) were divided into 3 groups ECV304(A), ECV-304 with radiation (B)and ECV-304 with active macrophage U937 and radiation(C). The survival rates, cell cycle and apoptosis of ECV-304 were determined 48 hours after irradiation. The concentrations of TGF-?1 and VEGF in the cultured supernatant were determined by ELISA method.The protein expression of the endothelial receptor KDR in ECV-304 cells was detected by immunofluorescence and flow-cytometry while its mRNA expression was detected by RT-PCR. Results After high dose of ion-irrdiation, the cellular proliferation activity decreased, apopototic cells increased and the cell ratio in G2/M phase increased.In addition,The secretion of VEGF and TGF-?1 was promoted with the increased KDR expression. Active macrophages could alleviate the decrease of cellular proliferation and the increase of apoptotic cells and cell ratio in G2/M phase induced by ion-irriation.Furthermore,the VEGF secretion and the KDR expression were enhanced and the secretion of TGF-?1 was inhibited by the active macrophages. Conclusions Ion irradiation could induce the decrease of cellular proloferation, the increase of apoptotic cells and the cells in G2/M phase.This might partly be related to the inhibiting roles of TGF-?1 secreted by the ECV-304 cells.Active macrophages might protect the endothelial cells from radiation injury.