Proliferative activity of bile of patients undergoing transduodenal sphincteroplasty / 中国普通外科杂志

ABSTRACT

Objective To explore the effects of bile in patients undergoing transduodenal sphincteroplasty (TSB) on the growth of human cholangiocarcinoma cell line QBC939 and the potential relation between transduodenal sphincteroplasty and cholangiocarcinoma. Methods TSB and normal bile (NB) sample were used for this study. The proliferative effect of bile was measured by using methabenzthiazuron (MTT) assay, and cell cycle and apoptosis were analyzed by using flow cytometry. Results Compared with NB,TSB significantly promote the proliferation of human cholangiocarcinoma QBC939 cells (P