Study of MEK2/ERK signal transduction pathway in the colorectal cancer / 中国普通外科杂志

ABSTRACT

Objective To study the role of MEK2/ERK signal transduction pathway in the development of colorectal cancer. Methods (1)Western blot analysis was performed on cancerous tissues and adjacent colonic tissues in 45 patients with colorectal cancers.(2)Human colorectal cancer cell line SW480 was treated with MEK inhibitor,and then MTT assay was used to measure the SW480 cells proliferation;and the expression of MEK2, p-ERK and C-myc in SW480 cells were measured by western blot. Results MEK2 protein level was increased in colorectal cancer compared with adjacent mucosa (P