Effect of the NF-?B inhibitor pyrrolidine dithiocarbamate on the apoptosis and proliferation of mesangial cells in the rat with anti-thymocyte serum nephritis / 中国病理生理杂志

ABSTRACT

AIM: To explore the effect of pyrrolidine dithiocarbamate (PDTC), the specific inhibitor of NF-?B, on anti-thymocyte serum nephritis (ATSN) in rats. METHODS: The rat model of ATSN was reproduced with rabbit anti-thymocyte serum (ATS). The rats were divided into ATSN group, ATSN+PDTC group and control group. The expression of NF-?B p65 and the apoptosis, lysis as well as proliferation of mesangial cells (MC) were examined by immunohistochemical staining, Tdt-mediated X-dUTP nick end labeling (TUNEL), light microscope and electron microscope at 40 minutes, 24 hours and 7 days after injection of ATS or normal serum. RESULTS: The expression of glomerular NF-?B p65 in the ATSN group was observed with significant difference compared to controls at 40 min (P