Content Determination of Ellagic Acid in Granati Cortex by HPCE / 中国药房

ABSTRACT

OBJECTIVE:To establish a method of high-performance capillary electrophoresis(HPCE)for content deter?mination of ellafic acid in granati cortex.METHODS:The capillary pillar used in this study was capillary without smear layer,the buffer solution was30mmol/L tromethamine-30mmol/L potassium dihydrogen phosphate(20∶9),the running voltage was20kV,the wavelength of detection was254nm,the column temperature was25℃,and the sampling condition was25mbar,5.0s.RESULTS:The detected concentration of ellagic acid showed a good linear relationship with the peak area score in the range of0.0398～0.3184mg/ml(r=0.9993).The average recovery rate was97.42%(RSD=1.84%).CONCLUSION:The present method is accurate and reliable,and it can be used for the quality control of granati cortex.