The effects of all-trans-retinoic acid on the proliferation and apoptosis of smooth muscle cells in human saphenous vein organ culture / 中国医师杂志

ABSTRACT

Objective To investigate the effects of all-trans-retinoic acid(atRA) on proliferation and apoptosis of smooth muscle cell in human saphenous vein organ culture.Methods Thirty segments(1cm) of human saphenous vein were divided into 3 groups HSV group,atRA group and control group.The human saphenous vein segments in atRA group and control group were maintained in organ culture for 14 days with 100?M atRA and equivalent concentrations of pure ethanol respectively.All 30 segments human saphenous vein were harvested and the level of proliferating cell nuclear antigen(PCNA) was detected immunohistochemically.The smooth muscle cell proliferation was observed pathologically.Results Cultured saphenous vein segments developed significant neointimal formation and marked smooth muscle cell proliferation(P