The effects of endothelin-1 and ACTH on the prolifertation of cultured normal human melanocytes / 中华皮肤科杂志

ABSTRACT

Objective To study the effects of endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH) on the proliferation of cultured normal human melanocytes. Methods Normal human melanocytes were obtained from the foreskins of healthy men, and cultured in minimum essential medium (MEM) with 10% bovine serum. The cultured melanocytes of the 3rd passage were treated with various concentrations of ET-1 and ACTH. Cell proliferation was assessed by the MTT method. Results ET-1 (0.1 ~ 1 000 nmol/L) promoted the proliferation of cultured human melanocytes. ACTH (10-13 ~ 10-9 mol/L) also promoted the proliferation of cultured human melanocytes (P