Simutaneous Assaying of Paracetamol and Caffeine in Ankahuangmin Capsules by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish HPLC method for the simultaneous assaying of paracetamol and caffeine in ankahua_ngmin capsules.METHODS:The determination was performed on phenomenex luna C18 column under room temperature;the mobile phase consisted of methanol-water(24∶76) with a flow rate at 1.0ml/min,detecting wavelength at 215 nm and sensibility at 0.01AUFS.RESULTS:The linear ranges for paracetamol and caffeine were 50～250?g/ml(r=0.9 999) and 5.0～25?g/ml(r=0.9 999),respectively.The average recoveries were 100.7%(RSD=1.8%)and 101.4%(RSD=1.4%),respectively.CONCLUSION:This method is simple,rapid and accurate,which can substitute for titration in the assaying of ankahua_ngmin capsules.