Construction of human prostate-specific membrane antigen expression vector and identification of tissue specificity / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
Dans Chinois
| WPRIM
| ID: wpr-528854
ABSTRACT
AIM:
To construct a prostate-specific expression vector of promoter and enhancer of human prostate-specific membrane antigen(PSMA).METHODS:
The promoter and enhancer of PSMA were amplified by PCR separately.The two segments were cloned into the expression vector pGL3-Basic,a prostate-specific expression vector pGL3-PSMP-PSME was constructed.The vector was transfected into prostatic carcinoma cell PC-3M and four kinds of non-prostatic carcinoma cells by lipofectamine.The activity of luciferase of transfected cells and tissue specificity of vector was examined at 48 hours after transfection.RESULTS:
With DNA sequence of the vector pGL3-PSMP-PSME,the segment of clone was proved correct.The activity of luciferase of the pGL3-PSMP-PSME was expressed distinctly in PC-3M and was not expressed in other nonprostate cell lines.CONCLUSION:
The prostate-specific expression vector was constructed successfully.It lays foundation for studying target gene therapy of the prostate cancer.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Chinese Journal of Pathophysiology
Année:
1986
Type:
Article
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