Relationship between viral gene activity and cytopathogenesis in human cytomegalovirus infection / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
de Zh
| WPRIM
| ID: wpr-529129
Bibliothèque responsable:
WPRO
ABSTRACT
AIM: To explore the relationship between human cytomegalovirus(HCMV) gene activity and cytopathogenesis in HCMV infection. METHODS: HCMV echelin-infected cell model was set up in vitro by coincubating passage cultured HEL and HCMV with different titers (group A: 10-5; group B: 10-3; and group C: 10-1). FQ-PCR was performed to evaluate the number of HCMV DNA copies. MCP mRNA was measured by RT-PCR. Meanwhile the development of cytopathologic effects (CPE) was observed under microscope and ultrastructural changes determined by TEM.RESULTS: Compared with group B and C in high HCMV titer, group A with low viral titer showed low HCMV DNA load (P
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Pathophysiology
Année:
1989
Type:
Article