Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-529286
ABSTRACT
AIM:
To investigate the effect of neuropeptide Y(NPY) on intracellular free calcium([Ca2+]i) and Ca2+ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS:
Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h.Fluorescent indicator Fluo-4 AM was used to detect [Ca2+]i and Fluo-5N AM was used to detect Ca2+ in sarcoplasmic reticulum(SR).Calcium image was recorded by laser scanning confocal microscope.The SR Ca2+ load was estimated by caffeine-induced Ca2+ transient(CCT).RESULTS:
24 h after incubation with NPY,compared with control group,the concentration of [Ca2+]i was significantly elevated(P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Pathophysiology
Année:
2000
Type:
Article
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