Preparation of Polyclonal Antibody of Recombinant Hirudin Ⅲ / 中国药房

ABSTRACT

OBJECTIVE:To prepare polyclonal antibody for recombinant hirudin Ⅲ (rHV3). METHODS: The antigens were prepared through two typical methods: one was conjugated with rHV3 itself, another was coupled with bovine serum albumin (BSA). Conjugation results were detected through electrophoresis. Then the rabbits, guinea pigs and rats were immunized and with serum sample taken for determination of antibody titer by double immunodiffusion test. RESULTS: Antibodies were produced in rabbits and DHP guinea pigs after immunization using rHV3-BSA as antigen, with antibody titer of 1∶16. CONCLUSION: After being immunized with rHV3-BSA, rabbits and DHP guinea pigs can produce polyclonal antibody for rHV3.