Determination of Thioctic Acid in Human Plasma by LC-MS / 中国药房

ABSTRACT

OBJECTIVE:An LC-MS method was developed for the determination of thioctic acid in human plasma.MET-HODSThe chromatographic separation was carried out on Zorbax SB C18 column with the mobile phase consisting of methanol-acetonitrile-0.1% glacial acetic acid(adjusted pH to 4.0 with ammonia)(55∶10∶35) at a flow rate of 0.3mL?min-1.Naproxen was used as internal standard.Column temperature was 40℃.SIM was used for determination of thioctic acid in human plasma.Ion mass spectral(m/z) of 205.0 was selected for thioctic acid and 229.3 for naproxen.RESULTS:The linear range of thioctic acid was 0.01～10?g?mL-1(r=0.999 9).The method recovery was within 97.61%～106.31%.The intra-day and inter-day RSD of thioctic acid(high,medium and low concentrations) were all less than 4%.CONCLUSION:The method is sensitive,accurate and reproducible,and suitable for the determination of thioctic acid in human plasma and its pharmacokinetic and bioavailability study.