Influence of IFN-? combined with M-pred on human embryonic lung fibroblasts / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
de Zh
| WPRIM
| ID: wpr-531216
Bibliothèque responsable:
WPRO
ABSTRACT
AIM:To look for a better method to deal with interstitial lung disease,interferon-gamma(IFN-?)combined with methylprednisolone(M-pred)to influence human embryonic lung fibroblast on proliferation,collagen synthesis and the expression of transforming growth factor-?1(TGF-?1)protein and mRNA were investigated.METHODS:Exponentially growing cells were preincubated for 48 h before harvested.The microculture tetrazolium(MTT)assay was used to measure the inhibition ratios of M-pred combined with different concentrations of IFN-?.The expression of proliferation cell nuclear antigen(PCNA)was detected by immunocytochemical analysis.Hydroxyproline kit was adopted to detect collagen synthesis.The expressions of TGF-?1 mRNA and protein were detected respectively by RT-PCR and Western blotting.RESULTS:Methylprednisolone,IFN-? as well as the combination of methylprednisolone and IFN-? inhibited the proliferation of HELF and the expression of PCNA in comparison with control group(P
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Pathophysiology
Année:
2000
Type:
Article