The exploration of STR genotyping of minute cells by MiniFiler kit / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6)2002.
Article
de Zh
| WPRIM
| ID: wpr-531276
Bibliothèque responsable:
WPRO
ABSTRACT
Objective Explore the feasibility of minute cells STR typing.Methods Minute cells which were obtained by micromanipulation method were amplified with MiniFiler kit,and detected by ABI 3130 genetic analyzer.Results Ten cells can be successfully genotyped.Complete genotyping results can be obtained for one,three or five cells but with stochastic effect.Conclusion Instability is observed in minute cells genotyping,so it can not be used in actual case work.Maybe increase the quantity of DNA template can improve the success rate.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Forensic Medicine
Année:
2002
Type:
Article