Effect of Kang Xianling decoction on TGF-?_1-Smad pathway in a unilateral ureteral obstruction rat model / 中国病理生理杂志

ABSTRACT

AIM: To study the effect of Kang Xianling decoction,comprised of dahuang,danshen,taoren,niuxi and danggui,on TGF-?1-Smad pathway in unilateral ureteral obstruction rat model.METHODS: Eighteen male SD rats were divided into 3 groups,sham group,model group and model group treated with Kang Xianling decoction randomly.Renal interstitial fibrosis model was established in rats by unilateral ureteral obstruction(UUO).After treatment for additional 14 d,parameters of hydroxyproline in obstructed kidney from 3 groups were analyzed.Rats were sacrificed and the pathological statuses of their kidneys were checked by HE staining and electron microscopy.Transforming growth factor-?1(TGF-?1) mRNA in kidney tissue was determined by RT-PCR.TGF-?1 receptor Ⅰ(T?RⅠ),TGF-?1 receptorⅡ(T?RⅡ),phosphorylated Smad2 and Smad2 protein were determined by Western blotting.RESULTS: Parameters of hydroxyproline in animals of model group were significantly increased than those in sham operation group(P