Simultaneous Determination of Main Components and the Related Substance ?-aminobutyric Acid in Al-endronate Tablets by Pre-column Derivatization HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
Dans Chinois
| WPRIM
| ID: wpr-531787
ABSTRACT
OBJECTIVE:
To develop a pre-column derivatization HPLC method for the simultaneous determination of the contents of alendronate and the related substance ?-aminobutyric acid in alendronate tablets.METHODS:
After AccQ pre-column derivatization,the sample was determined with Diamonsil C18 column used as stationary phase and acetonitrile-35 mmol?L-1 acetate buffer solution(13.5∶86.5,V/V) as a mobile phase.The flow rate was 0.7 mL?min-1 and the column temperature was set at 35 ℃.RESULTS:
Alendronate and ?-aminobutyric acid were well-separated and able to be determined simultaneously,with their linear ranges at 30.9~990 ?g?mL-1(r=0.999 6) and 0.307~960 ?g?mL-1(r=0.999 9),respectively and their average recovery rates at 99.3~100.8% and 99.3~100.2%,respectively.The intra-day RSDs of both were no more than 0.8% and their inter-day RSDs were no more than 1.9%.CONCLUSIONS:
The method is simple,selective and suitable for the determination of alendronate and the related substance ?-aminobutyric acid of alendronate tablets.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
2001
Type:
Article
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