Separation,Purification and Detection of Product of Bovine Hemoglobin Modified by Polyethylene Glycol / 中国药房

ABSTRACT

OBJECTIVE: Researching the appropriate methods to separation,purification and detection the mixture of PEG modifier SC-mPEG with BHB.METHODS: Adopting cation-exchange chromatography and organic solvent precipitation to separate the mixture,then detecting it by SDS-PAGE and TNBS methods.RESULTS: Modifer and non-modifer product were separation by cation-exchange chromatography.The average modification rates of two peak product from cation-exchange chromatography are 52.95% and 14.19% detected by TNBS methods.CONCLUSIONS: Cation-exchange chromatography methods could be to separate and purify the SC-mPEG-BHB,TNBS methods coule be to detect the average modification rate of the SC-mPEG-BHB.