Long-term Toxicity of Repeated Intramuscular Injection of Escherichia Coli O157:H7 Polysaccharide-conjugated Vaccine in Rats / 中国药房
China Pharmacy
;
(12)2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-533058
ABSTRACT
OBJECTIVE:
To investigate the long-term toxicity of repeated intramuscular injection of Escherichia coli O157∶H7 polysaccharide-conjugated vaccine(O157) in rats so as to provide safety evidence for clinical trials.METHODS:
A total of 48 SD rats were randomly assigned to receive either 0.5 mL vaccine(containing 25 ?g polysaccharides) (immunization group,n=24) or phosphate buffered solution (PBS,control group,n=24) with the same volume for 3 times at a dose interval of two weeks.Sacrifice of 6 rats in each group were scheduled at 2 weeks after first immunization,and at 1,3,and 5 weeks after the third immunization,respectively for observation and determination of hematological and biochemical parameters,histopathology,specific antibody,myeloid tissue,the tissues in injection sites,etc.RESULTS:
Compared with control group,immunization group showed no significant pathological change except the dynamic regular change of some hematological parameters induced by the immunization,and no immunologic system damage was observed.CONCLUSION:
Repeated intramuscular injection with O157 vaccine in rats wouldn't cause overt toxicity and local irritation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
China Pharmacy
Année:
2007
Type:
Article
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