Effect of NF-?B inhibitor pyrrolidine dithiocarbonate on the proliferation and apoptosis in K562 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-533237
ABSTRACT
AIM:
To investigate the effect of pyrrolidine dithiocarbamate (PDTC),a specific inhibitor of NF-?B on the proliferation and apoptosis of K562 cells and to explore the anti-tumor mechanism of PDTC.METHODS:
Trans AMTM NF-?B p65 kit was used to detect the activity of p65 in K562 cells treated by PDTC. The effect of PDTC on the proliferation of K562 cells was measured by WST-1 method. DNA damage was detected by single cell gel electrophoresis (comet assay). The procaspase-3 and activated protein level of caspase-3 were detected by Western blotting.RESULTS:
The activity of p65 in K562 cells was inhibited after treated by PDTC (P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Pathophysiology
Année:
2000
Type:
Article
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