In Vitro Inhibitory Effect of Wogonin on Human Osteosarcoma / 中国药房

ABSTRACT

OBJECTIVE:To study the inhibitory effect of wogonin on human osteosarcoma. METHODS:MTT chromatometry was employed to determine the the influence of wogonin to the activity of U2OS cell line and its time-dependent effect and dose-dependent effect against osteosarcoma cell line detected. The apoptosis of wogonin-treated U2OS cells was determined by flow cytometry together with morphological observation,meanwhile the cell cycle of U2OS cell line was analyzed. RESULTS:The growth of U2OS cells was inhibited and the apoptosis of which was promoted by wogonin in a time-dependent and dose-dependent manner. The U2OS cells were arrested at G0/G1 phase with conspicuous apoptotic peak,nuclear fragmentation and chromosome agglomeration. The IC50 against U2OS cells stood at (37.18?1.57) ?mol?mL-1. CONCLUSION:Wogonin can inhibit cell growth and promote apoptosis of U2OS cells.