Simultaneous Determination of Anethole Trithione and Its Metabolite in Plasma of Beagle Dogs by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an HPLC method for the determination of anethole trithione(AT)and its metabolite 4-hydroxy-anethole trithione(ATX)in plasma of beagle dogs simultaneously. METHODS:Protein in plasma was deposited directly using acetonitrile and analyzed by supernate. The separation was performed on Diamonsil ODS-C18 column with a mobile phase canodn sAisTtiXng woefr em ectohmanpolel tealnyd swepaaterar(ted8 0w∶i2t0h)ouat t ian teflrofewr ernactee . oTf h1e. 0r emteLn?timoni nt-im1 we iwtha sth 5e. 5d emteicnt iaonnd w 3a.v4e mleinng trhe sopfe c3t4iv6e nlym. .T RheE SliUneLaTr SranAgTe were 0.254~3.392 ?g?mL-1(r=0.999 9)for AT with average recovery of(93.89?2.39)% and 0.125～1.664 ?g?mL-1 for ATX (r=0.999 8)with average recovery of(96.28?2.83)%. The intra-day RSD and inter-day RSD of AT were 0.83%～4.11% and 0.71%～4.51% while that of ATX were 0.55%～1.23% and 1.74%～3.92%,respectively. The detection limit of AT in human plasma was 0.254 ?g?mL-1 and that of ATX was 0.125 ?g?mL-1. CONCLUSION:It is proved that established method is simple,accurate and reproducible for plasma concentration determination of AT and ATX in plasma of beagle dogs.