Content Determination of Paenol in Liuwei Dihuang Soft Ectract by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To establish the HPLC method for the content determination of paenol in Liuwei dihuang soft extract. METHODS: The octadecylsilyl silicagel was used as filter. Mobile phase consisted of methanol-water(70 ∶ 30) at detection wavelength of 274 nm. The flow rate was 0.8 mL?min-1 and the column temperature was room temperature. RESULTS: The linear range of paeonol was 50～300 ?g?mL-1(?g?mL-1) with an average recovery of 99.63%(RSD=1.26%, n=6). CONCLUSION: This method is sensitive, accurate and reproducible for quality control of Liuwei dihuang soft extract.