An ELISA Assay to Detect IgM and IgA Anti-LSP / 中国免疫学杂志

ABSTRACT

An indirect ELISA to detect IgM and IgA autoantibodies against liver-specific membrane lipoprotein(anti-LSP)in human sera is described. Human LSP was used as the coated antigen. Serum samples were pre-absorbed by SPA(bacteria)reagent. The results of serial identifications and the detection of 182 patiens with viral hepatitis indicated this procedure was specific, its duplicate was satisfactory, and the method could be valuable for iurther studying the role of anti-LSP in the pathogenesis of viral hepatitis. Key words ELISA, Liver-specific membrane lipoprotein, Viral hepatitis, IgM, IgA.