Enzyme Immunoassay of Thyroid-stimulating Hormone And Its Clinical Use / 中国免疫学杂志

ABSTRACT

A method for enzyme immunoassay of thyroid-stimulating hormone(TSH) was presented. Horse-redish peroxidase was used as the marker enzyme. The separation of the bound and free fractions was achieved by second anti body coated polyacetyl beads. Para-cresollH_2O_2 system was used as the substrate and the enzyme activity was measured fluorophotometrically. The assay sensitivity is 0.08 ?U/tube. The volume of serum used is 20-50?l. Within assay C. V. was 7.7-11.7％. Inter-assay C. V. was 4.8-17％. The TSH values detected by this method correlates well with that of RIA(r=0.91). Three principal reagents(anti-TSH serum, second antibody coated polyacetol beads and HRP-TSH conjugate) were all generated by the laboratory. According to the normal values investigated, the hypothyroid samples tested and TRH releasing tests, the results were applicable to the clinical conditions very well. The benefits of EIA versus that of RIA have been discussed.