Double blind controlled stuby for detection of anti-LSP by RIA and ELISA / 中国免疫学杂志

ABSTRACT

0.05).The sera of 90 patients were positive or negative detectedsimultaneously by RIA and ELISA,and the concordance rate of both methods was79.7%.The sera of 18 patients were positive detected by RIA and negative by ELISA,and the sera of 5 patients were positive detected by ELISA and negative by RIA,sothe inconsistent rate of both methods were 15.9%and 4.4% respectively.Based onthe literatures reviewed,the authors suggested that the causes of inconsistent resultsin various laboratories might be related to following factors,1.different sensitivitybetween various methods for deermination;2.the diffences between experimental conditions;3.the antigens used in the test from different species;4.the blood specimens collected at different period of the course.