Study on the Effects of Fluorosis on Rat Renal Oxidative Stress, Necrosis,Apoptosis and Proliferation / 环境与健康杂志

ABSTRACT

Objective This study was conducted to explore the effects of fluorosis on rat renal apoptosis and proliferation, oxidative stress and necrosis. Methods Wistar rats were provided with distilled water containing NaF(50mg/L) for six months. Kidney cell apoptosis and the cell cycle of proliferation were detected by TUNEL (TdT-mediated dUTP Nick End Labelling) and flow cytometry. Rsults TUNEL_positive cells could be detected in fluoride_treated rat kidney. The apoptotic rates of fluoride_treated kidney cells were higher than that of control significantly. Fluorosis could reduce the cell number of G2/M period in cell cycle and decrease DNA relative content significantly. In addition, fluorosis could induce rat renal oxidative stress and necrosis. Cnclusion It was suggested that fluorosis could induce apoptosis and change the cell cycle of rat renal cells in vivo, and also could result in rat renal oxidative stress and necrosis.