Expression and specific cell response of autoantigen Dsg3 in pemphigus vulgaris / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
Dans Chinois
| WPRIM
| ID: wpr-536666
ABSTRACT
Objective:
In view of analysis of role of autoantigen Dsg3 in specific T cell response to understand the molecular mechanism of autoimmune diseases.Methods:
Based on the sequence analysis of autoantigen Dsg3,the five fractions cDNAs of Dsg3 were cloned and Dsg3-GST fusion proteins induced by IPTG in E.coli.JM109 were purified,then mixed-cultured with T cell from PV patients.The T cell proliferation response were analyzed by MTT.Results:
Dsg3 E1,E2 and E4,E5 stimulated T cell from PV patients,not controls.Conclusion:
Dsg3 E1,E2 and E4,E5 contained the epitopes relevant to T-B cell interaction,which play an important role in the pathogenesis of pemphigus vulgaris.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Immunology
Année:
1985
Type:
Article
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