Regulation of rhRPL on immunoactivity of NK cell lines / 中国免疫学杂志

ABSTRACT

Objective: To explore the effects of rhPRL on the cytotoxicity and proliferation of natural killer cell lines. Methods: Cytotoxici-ty and proliferation analysis were done by MTT,RT-PCR method,direct immunofluoresence technique and FAGS protocol was applied to detect the PRL-R and CD25 expression on NK cell lines. Results: MTT results indicated that PRL play a significant roles on the proliferation and cytotoxicity of IL-2 dependent cell lines NKL and NK-92 in the presence of trace amounts of IL-2. In contrast, PRL did not significantly alter the proliferation and cytolyric activity of YT. RT-PCR and FACS analysis showed three NK cell lines expressed PRL-R and CD25 differently, stimulation of rhPRL could upregulate CD25 and IL-2 expression. Conclusion: rhPRL could augument the proliferation and cytotoxicity of NK cell partly by upreglating of IL-2. Ra chain(CD25) and interaction with IL-2 signal transduction.