Effects of Dayecai (Selaginella doedealeinii) and Chinese Livistona (Livistona chinensis)on the activity of Protein Kinase C / 中草药

ABSTRACT

Protein Kinase C (PKC ) was partially purified from rat brain by DEAE-52 column chromatography, and the effects of ethanol extracts of Selaginella doederleinii and Livistona chinensis on PKC activity were investigated.In order to verify the reliability of the assay system, the effect of polymyxin B, a known PKC inhibitor, on the activity of PKC was also tested. Results showed that the ethanol extract of S. doederleinii strongly inhibited PKC activity with IC50 2.2?g/ml. The ethanol extract of L. chinensis (100?g/ml ) inhibited PKC activity by 66.6%. Polymyxin B inhibited PKC activity with IC5017.7 U/ml.