Construction of specific ribozymes against second exon of ?_1 Ⅰ and ?_1 Ⅲ procollagen genes / 中华医学美学美容杂志

ABSTRACT

Objective To construct two ribozyme genes (gⅠand gⅢ) by using PCR according to the requirement of ribozyme's domains and the nucleotide sequences of ?_1Ⅰand Ⅲ procollagen genes. Methods First we constructed two ribozyme genes (g, gⅢ) by using PCR, and then separately cloned into the downstream of T7 promoter of pGEM-T vector. Results The resulting recombinant plasmids (pT-gⅠ, pT-gⅢ) were verified by restriction enzymes, PCR and sequencing. Conclusion The expected two ribozyme genes are constructed successfully and this work affords the basis for the preparation of large amounts of ribozyme molecules and for the study on interaction between mRNA and ribozyme in vivo.