Study of nitric oxide during ischemia-reperfusion of kidney transplantation in rats using electron paramagnetic resonance / 中华泌尿外科杂志

ABSTRACT

Objective To monitor the production of nitric oxide(NO)and to study its role during ischemia-reperfusion of kidney transplantation in rats using electron paramagnetic resonance(EPR). Methods LEW male rats (age of 8 to 10 months,body weight of 200 to 230 g) were included.Of them 30 were assigned to donor group whose kidney grafts were stored at 0℃ for 24 hours.The remaining 45 rats were equally divided into 3 groups (15 in each group).Group 1 served as controls.In Group 2,allotransplantations of kidneys were performed,and both original kidneys were removed during reperfusions.In Group 3,2 hours prior to the operation,the donors and the recipients were both treated with L-NAME at the dose of 30 mg/kg.Reperfusions were done to the transplanted kidneys and both original kidneys were removed.The production of NO was measured with ERP at each time point before and after blood flow recovery.The creatinine level,GFR and the tissue carbonyl content were recorded on the first and fifth days after operation. Results In Group 2,the production of NO remarkably increased after 15 minutes' reperfusion and kept increasing for 120 minutes. Then the level fell to normal after 210 minutes.In Group 3,the recorded creatinine levels were higher than those in Group 2 at the 24th hour ( P