Construction of recombinant nonstructural P_2C plasmid in coxsackie B4 virus / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-541584
ABSTRACT
Objective:
To construct the recombinant nonstructural P_2C plasmid in coxsackie B4 virus.Methods:
Using the RT-PCR,we fishing the nonstructural protein P_2C cDNA fraction from Coxsackie B4 virus(CVB4) and cloning into the PUCm-T vector, then transfer it into E.coli to construct the recombinant plasmid.Results:
Using the total RNA of Coxsackie B4 virus as model, we amplied 987 bp fraction and cloned into PUCm-T vector identifying with BamH Ⅰ and Hind Ⅲ double enzyme.Its products were consistent with nonstructural protein P_2C PCR productions.Conclusion:
The recombinant nonstructural P_2C plasmid was constructed succesfully in coxsackie B4 virus.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Immunology
Année:
2000
Type:
Article
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