The expression of COX-2,PCNA and Cyclin D_1 in the model of reflux esophagitis / 西安交通大学学报(医学版)

ABSTRACT

Objective To investigate the expression of COX-2,PCNA and Cyclin D_1 in the progress of reflux esophagitis.Methods A total of 54 SD rats were divided into two groups randomly model group were treated with esophagoduodenostomy;control group were normals.The lesions of esophageal mucosa were observed in the 5~(th),17~(th),28~(th),40~(th) week in model group and 40~(th) week in control group,respectively.The changes of COX-2,PCNA and Cyclin D_1 were evaluated by immunoperoxidase staining in the progress of reflux esophagitis.Results The positive rates of COX-2,PCNA and Cyclin D_1 were 0.0%,0.0% and 12.5% in normal respectively,and were(42.1%),42.1% and 42.1% in RE respectively,73.7%,63.2% and 63.2% in BE,and 100.0%,100.0% and 100.0% in EAC,respectively.The expression of COX-2,PCNA and Cyclin D_(1) increased gradually with the development of reflux esophagitis,and significant difference was found between RE,BE and EAC,no difference was found between BE and EAC.Conclusion In the progress of RE,COX-2,PCNA and Cyclin D_1 increased gradually.