Effects of IGF-1 on the biological characteristics of mouse dental follicle cells / 实用口腔医学杂志

ABSTRACT

Objective:To study the effects of IGF-1 on the proliferation,total protein amount and cytodifferentiation of mouse dental follicle cells.Methods:The dental follicle cells of passage 3 were incubated with different concentrations((0.005),0.01,0.05,0.1 and 0.5 mg/L)of IGF-1 respectively,cell proliferation,alkaline phosphatase(ALP),total protein amount were measured by MTT assay and spectrophotometer respectively.Effects of 0.05 and 0.1 mg/L IGF-1 on mineralization potential were studied by Von Kossa staining.Results:IGF-1(ml/L) at 0.005~0.1 increased the proliferation and total protein amount(P